Electrical and Computer Engineering
ECE Neuroengineering Seminar Series
Friday, November 15, 2013
Interfacing Cells and Biomolecules with Floating Gate Ion Sensitive Transistors
to 2:00 PM
A227 Abercrombie Engineering Laboratory
6100 Main St
Houston, Texas, USA
In this talk, I will describe CMOS compatible transistor interfaces to cells and biomolecules with an emphasis on innovative device architectures and improved functionality. I will introduce the concept of a Programmable ISFET which functions on the principle of “Electrochemical Gating”, a mechanism by which the exposed electrode/electrolyte interface charge can be electrically controlled. I will emphasize through both simulation and experiment that this effect leads to surface pH tuning which helps establish a single transistor sensor/actuator hybrid. I will then describe our recent experiments on cell-transistor interfaces with a focus on high temporal electrochemical recordings of exocytosis and stimulus secretion coupling from electrically excitable and non-excitable cells, further corroborated by fluorescent readout. Through both experiment and theory I will show that in addition to transmitter release the transistor recordings are sensitive to ion channel distributions across the cell membrane. Time permitting, I will switch direction and briefly explain our results on measuring DNA interactions with the capability of on demand DNA adsorption and release. I will then conclude by suggesting ways of improving molecular sensitivity specifically through Debye layer modification and dielectric spectroscopy.
Host: Jacob Robinson
Biography of Krishna Jayant:
Krishna Jayant completed his Bachelors in Electrical and Electronics Engineering from the National Institute of Technology Tiruchirappalli, India in 2005. From 2005to 2006 he was a research assistant at the Indian Institute of Science, Bangalore, India where he worked in the area of RF MEMS. In 2006 he joined the Advanced Research Center for Electronic Systems (ARCES) at the University of Bologna Italy, where he worked on Dielectrophoresis based Cell on Chip systems for isolation and manipulation of rare cancer cells. In the spring of 2008 he started his PhD under the supervision of Prof. Edwin C. Kan at Cornell where he investigated FET based cell and biomolecular sensors focused on detecting stimulus secretion coupling and molecular interactions. In 2012 he held a visiting PhD position at the Kavli Institute of Nanoscience at TU Delft where he worked in the group of Prof. Cees Dekker on graphene nanopores. His current research interests include combined electrical and optical neural interface technologies, neural circuit reconstruction, single molecule biophysics, nano-electrochemistry, solid-state devices and integrated CMOS sensors.